The ionic liquid isopropylammonium formate as a mobile phase modifier to improve protein stability during reversed phase liquid chromatography

Isopropylammonium formate as a mobile phase modifier for liquid chromatography.

Isopropylammonium formate (IPAF), a new alkylammonium formate (AAF) room temperature ionic liquid, has been synthesized from isopropylamine and formic acid and characterized as an organic solvent mobile phase replacement for reversed-phase liquid chromatography (LC). Characterization of IPAF solvent properties in water such as pH, conductivity, and viscosity, as well as its synthesis, is descri...

Effect of Ionic Liquid as Mobile-phase Modifier

Studies have been performed to investigate the effect of an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate, as mobile-phase modifier on the retention behaviour of N-carbobenzyloxy-D-phenylalanine and D-tryptophan on a reversed-phase silica-based column. Retention of these amino acids was determined with water–acetonitrile mobile phases and with mobile phases containing 2, 5, or 10 ...

Normal-phase liquid chromatography of plant hormones using reversed cholic acid micelles as the mobile phase.

When several plant hormones with similar structures are present in a sample, conventional analytical methods are not sufficiently selective to effectively differentiate them. However, the excellent selectivity of micellar liquid chromatography allows similarly structured compounds to be easily differentiated. We used reversed cholic acid micelles dissolved in tetrahydrofuran as the mobile phase...

Reversed-phase high-performance liquid chromatography.

EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS

The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...